Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

HONG Xin-Guo; WENG Yu-Xiang; LI Ming

Chinese Physics C> 2003, Vol. 27> Issue(S1) : 57-62

Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100039,China2 Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences，Beijing l00080,China

Abstract
HTML
Reference
Related

PDF

Abstract：
The low-resolution shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter sphaeroides 2.4. 1 in detergent solution has been determined from synchrotron small-angle X-ray scattering (SAXS) data using directly curve fitting by an ellipsoid and ab initio shape determination method or multipole expansion respectivelv. The results indicate that the LH2 proteins in aoueous solution has a shape of oblate plate. In contrast to the perfect symmetrical ring-like structure of LH2 in crystalline, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ring-like crystal structure, which is consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.

References

[1]

Mayor U , Guydosh N R , Johnson C M et al. Nature, 2003 ,421 :863-8672 Sardet C, Tardieu A, Luzzati V. J. Mol. Bio. , 1976,105:383-4073 Osborne H B, Sardet C, Michel-Villaz M et al. J. Mol. Biol. ,1978,123 : 177-2064 Svergun D I. J. Appl. Cryst. ,2000,33:530-5345 Svergun D I , Stuhrmann H B. Acta Cryst. ,1991, A47:736-7446 Svergun D I , Volkov V V, Kozin M B et al. Acta Cryst. , 1996, A52 :419-4267 Grossmann J G, Hasnain S S. J. Appl. Cryst. , 1997,30:770-7758 Ockwell D M, Hough M A, Grossmann J G et al. Acta Cryst. ,2000, D56 : 1002-10069 HAo Q. Acta Cryst. , 2001, D57:1410-141410 LNLS Activity Report, 200111 van Grondelle R, Dekker J P, Gillbro T et al. Biophys. Acta. ,1994, 1187: 112 Pullerits T, Sundstrm V. Ace. Chem. Res. , 1996 , 29 , 381-38913 Fleming G R, van Grondelle R. Curr. Opin. Struct. Biol. ,1997,7 :73814 McDerniott G, Prince S M , Freer A et al. Nature, 1995 , 374 : 517-52115 Freer A . Prince S, Sauer K et al. Structure, 1996,4 :44916 Sundstrom V, Pullerits T, van Grondelle R. J. Phys. Chem. B. ,1999,103 :2327-234617 HU X , Schulten K. Phys. Today, 1997, 50 : 28-3418 XIE S, Trautman J K. Annu. Rev. Phys. Chem. ,1998,49: 441-48019 Ropp M A, Sytnik A, Howard T D et al. Proc. Natl. Acad. Sci. ,1999,96:11271-1127620 Antoine M, van Oijen A M, Ketelaars M et al. J. Science, 1999,285 :400-40221 Ketelaars M, van Oijen A M, Matsushita M et al. Biophys. J. ,2001, 80:1591-160322 Matsushita M, Ketelass M , van Oijen A M et al. Biophys. J. ,2001,80: 1604-161423 Ohashi N , Kochi N , Kuki M et al. Biospnclroscopy , 1996,2 :59-6924 Dong B Z et al. J. Appl. Cryslallogr. ,1997,30:87725 Guinier A, Fournet G. John Wiley Sons : London, 195526 Glatter O , Kralky 0. Acadeniic Press, NPW York, 198227 Svergun DI, Barheralo C , Koch M H. J. Appl. Cryslallogr. ,1995, 28 :768-77328 Snninnyuk A V , Svnrgun D I. J. Appl. Cryst. , 1991, 24:537-54029 Svergun D I. J. Appl. Cryst. ,1992, 25: 495-50330 Stuhrniann H B. Acla Cryst. ,1970, A26 :297-30631 Press W H, Tnukolsky S A. 1992, Universily Press, Camhridge32 HONC Xin-Guo, WENC Yu-Xiang, LI Ming, Accepted by Biophys.J. (in press)33 Roth M , Arnoux B , Ducruix A. Reiss-Husson , F. Biochnmistry,1991,30:9430-941334 Prince S M, Howard T D, Myles A A et al. J. Mol. Biol. , 2003,326 :307-31535 Le Maire M, Kwee S, Andnrsen J P, Moller J V. Eur. J. Biochem.,1983, 129 :525-53236 Roth M, Lewit-Bentley M , Michel H et al. Nature, 1989, 340:65-66227 Roth M , Arnoux B , Ducruix A et al. Biochnmistry, 1991 , 30 :9403-9413

[1]	PING Rong-Gang , MO Xiao-Hu , DU Shu-Xian , LIU Jing , MA Lian-Liang , SHEN Cheng-Ping , WANG Zhi-Yong , YUAN Chang-Zheng . Extract Signals by Fitting χ² Distribution of the Kinematic Fit. Chinese Physics C, 2007, 31(3): 229-231.
[2]	LIU Ying-Bin , GAO Yu-Xi , CHEN Chun-Ying , LI Bai , YU Hong-Wei , WANG Jiang-Xue , HE Wei , HUANG Yu-Ying , CHAI Zhi-Fang . Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation. Chinese Physics C, 2005, 29(S1): 61-64.
[3]	LI Shi-Hong , JIANG Shan , HE Ming , GUAN Yong-Jing , DONG Ke-Jun , WU Shao-Yong , YUAN Yuan , MI Sheng-Quan , LIU Shu-Tian , YUAN Jian . Measurements of 41Ca in Biological Samples by AMS Using CaF2 Target. Chinese Physics C, 2005, 29(12): 1210-1213.
[4]	HU Shu-Xin , LI Da-Peng , MAI Zhen-Hong , LI Ming . Small Angle X-ray Scattering Study of the Influence of Temperature on the Structure of DNA/Lipid Complexes. Chinese Physics C, 2005, 29(S1): 4-7.
[5]	SHEN Da-Wa , YU Hui , WU Zong-Hua , HUANG Yong . The Morphology of CDA-g-PMMA Copolymers in Solution Studied by SR-SAXS. Chinese Physics C, 2005, 29(S1): 8-11.
[6]	XU Yan-Bing . Influence of 1h_11/2 Orbit on the Shapes of Even Even Nuclei Around A～100 Region. Chinese Physics C, 2004, 28(6): 601-606.
[7]	LI Shu-Jun , ZHAO Wei , CHU Wang-Sheng , NIU Li-Wen , TENG Mai-Kun , HU Tian-Dou , XIE Ya-Ning , WU Zi-Yu . XAS Study of a Venom Metalloprotein. Chinese Physics C, 2003, 27(S1): 19-22.
[8]	HONG Xin-Guo , DU Lu-Chao , YE Man-Ping , WENG Yu-Xiang , LI Ming . In Situ Synchrotron Small Angle X-ray Scattering Studies of TiO₂ Colloid at Elevated Temperature. Chinese Physics C, 2003, 27(S1): 63-66.
[9]	Medina Ablikim , HUANG Wei-Cheng , . New Realization of N=2 Supersymmetric QuantumMechanics and Shape Invariance. Chinese Physics C, 2002, 26(4): 338-345.
[10]	ZHAO Hui , LI Zhi-Hong , DONG Bao-Zhong , RONG Li-Xia , WANG Jun . Preliminary Transformation on Experimental Data of Small Angle X-Ray Scattering. Chinese Physics C, 2001, 25(S1): 81-84.
[11]	SHENG Yi , LI Xiao-Wei , LI Dong-Mei , TAO Ye . Study on Vacuum ltraviolet Circular Dichroism Spectra of Several Proteins. Chinese Physics C, 2001, 25(S1): 66-69.
[12]	Xu Yanbing , Liu Yingtai , Liao Jizhi . Systematic Research on Shapes of Even Even Nuclei Around A～100 Regions With DHF Method. Chinese Physics C, 1999, 23(6): 561-566.
[13]	Pan Qiangyan , Luo Yixiao , De Poli , de Angelis . Band Crossings and Shape Changes in the K^π=5－/2 Band in ⁷⁹Kr. Chinese Physics C, 1998, 22(3): 209-216.
[14]	Dong Baozhong , Sheng Wenjun , Li Guangcheng , Zhang Zhijie . Small Angle Scattering Station of BEPC Synchrotron Radiation Laboratory. Chinese Physics C, 1995, 19(3): 284-288.
[15]	Wang Jun , Cui Mingqi , Xu Wenxuan , Huang Yuying , Wang Dewu , Liu Jianfei , Xian Dingchang . Study on Structure of Multilayers by Synchrotron Radiation Small Angle Diffraction Method. Chinese Physics C, 1994, 18(1): 13-19.
[16]	CHEN Xue-Jun , ZHENG Liang-You . Multiple-Scattering Expansion for Quasi-Elastic Knockout Reaction (p,2p). Chinese Physics C, 1992, 16(1): 68-74.
[17]	ZHANG Jie , JIANG Da-Zhen , YANG Jin-Qing , ZHANG Ying-Ji , HE Jian-Hua , FU De-Ji , CHENG Xiao-Wu . Study of Low-Lying States in ^58,60,62,64Ni by (p,p′) Scattering at E_p=25.1MeV for 2+1 State and IBA Analysis for Spectra and B(E2) Ratios. Chinese Physics C, 1991, 15(9): 836-843.
[18]	BIAN Jian-Guo . A REAL-SPACE RENORMALIZATION-GROUP ANALYSIS FOR Z₂ LATTICE GAUGE THEORY COUPLED TO A HIGGS FIELD. Chinese Physics C, 1989, 13(1): 29-34.
[19]	SONG Xiao-Tong , DAI You-Shan . ELECTROMAGNETIC FORM FACTORS OF (0—1/2) ELECTROMAGNETIC BOUND SYSTEM. Chinese Physics C, 1985, 9(3): 371-376.
[20]	Lu Jing-xian . 2π→2K SCATTERING AND πK ELASTIC SCATTERING. Chinese Physics C, 1980, 4(1): 28-32.

Access

Get Citation

HONG Xin-Guo, WENG Yu-Xiang and LI Ming. Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering[J]. Chinese Physics C, 2003, 27(S1): 57-62.

RIS(for EndNote,Reference Manager,ProCite)

BibTex

Txt

Milestone

Received: 2003-11-21
Revised: 1900-01-01

Article Metric

Article Views(3268)
PDF Downloads(591)
Cited by(0)

Policy on re-use

To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

Corresponding author: HONG Xin-Guo,

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100039,China2 Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences，Beijing l00080,China

Received Date: 2003-11-21

Accepted Date: 1900-01-01

Available Online: 2003-01-02

Abstract: The low-resolution shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter sphaeroides 2.4. 1 in detergent solution has been determined from synchrotron small-angle X-ray scattering (SAXS) data using directly curve fitting by an ellipsoid and ab initio shape determination method or multipole expansion respectivelv. The results indicate that the LH2 proteins in aoueous solution has a shape of oblate plate. In contrast to the perfect symmetrical ring-like structure of LH2 in crystalline, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ring-like crystal structure, which is consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.

HTML

Reference (1)

Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering

Abstract：

References

Access

Article Metrics

Metrics

通讯作者: 陈斌, bchen63@163.com

Email This Article